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This transcript serves as a template for the next step of protein biosynthesis, translation, through the help of the enzyme, RNA polymerase. a copy of the coding sequence for a particular protein) is produced, generally by transcribing the template strand of the DNA. Transcription is a biological process wherein the mRNA transcript (i.e. capping, polyadenylation, and splicing) ensue. In eukaryotes, post-transcriptional events (e.g. Finally, hydrogen bonds of the RNA-DNA helix break in order to release the newly synthesized mRNA transcript. RNA sugar-phosphate backbone forms on the RNA strand. RNA polymerase adds RNA nucleotides as it traverses the DNA template. RNA polymerase, then, escapes the promoter. Abortive initiation causes the formation and the release of small RNA products. A transcription bubble forms, opening the DNA strand and exposing a segment that will be transcribed. During initiation, RNA polymerase binds to the promoter region of the DNA. 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